Table 8. Effect of replacing methionine with different levels of betaine and choline (mg/kg) on blood liver enzymes and status of antioxidative factors in broilers (Trial 1).
| Treatments | ||||||||
|---|---|---|---|---|---|---|---|---|
| Parameter | Control- | Control + | 280 Chol | 560 Chol | 320 Bet | 640 Bet | SEM | P-value |
| ALP (U/L) | 5373 | 4576 | 5741 | 5063 | 5365 | 4666 | 298.8 | 0.8790 |
| AST (U/L) | 222.9 | 250.7 | 263.5 | 258.7 | 267.9 | 263.6 | 8.35 | 0.5961 |
| ALT (U/L) | 10.5 | 8.9 | 9.1 | 11.4 | 9.6 | 11.5 | 0.83 | 0.9153 |
| TAS (mmol/L) | 1.32b | 1.70a | 1.22b | 1.27b | 1.48ab | 1.30b | 0.047 | 0.0179 |
| SOD (U/g Hb) | 100.1 | 97.7 | 120.5 | 102.4 | 118.2 | 94.7 | 4.19 | 0.3586 |
| GPx (U/g Hb) | 42.9b | 48.7b | 49.0b | 49.5b | 70.7a | 55.2ab | 2.56 | 0.0310 |
| MDA (nmol/mL) | 1.86 | 2.04 | 2.29 | 1.89 | 1.90 | 1.83 | 0.072 | 0.5020 |
| Uric acid (mg/dL) | 2.28b | 3.71a | 1.91b | 2.77ab | 2.71ab | 2.41b | 0.151 | 0.0079 |
| Hb(g/dL) | 10.4 | 11.3 | 10.6 | 11.1 | 11.0 | 11.2 | 0.18 | 0.6186 |
| SAA (µg/mL) | 2.63 | 2.91 | 3.03 | 3.03 | 3.08 | 2.90 | 0.083 | 0.6545 |
| CEP (µg/L) | 1486 | 1461 | 1470 | 1510 | 1389 | 1384 | 21.8 | 0.4953 |
ALP, alkaline phosphatase; AST, aspartate amino transferase; ALT, alanine amino transferase; TAS, total antioxidant status; SOD, superoxide dismutase; GPx, glutathione peroxidase; MDA, malondialdehyde; Hb, hemoglobin; SAA, serum amyloid A; CEP, ceruloplasmin; Chol, choline; Bet, betaine.
a–c Mean values within a row with different superscripts differ significantly (P<0.05). Values represent 4 replicates of 2 birds per replicate for each treatment.