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. 2018 Apr 25;55(4):288–300. doi: 10.2141/jpsa.0170217

Table 1. Primer sequences of TLRs, cytokines, and RPS17 for PCR.

Target genes Sequences 5′-3′ Accession No.
TLR1-1 F: AGGTTGGACTTCTTATTGAGGCATAC
R: AGATGAATCCCAAACTAGCAGAAAAA NM_001007488
TLR1-2 F: AGTCCATCTTTGTGTTGTCGCC
R: ATTGGCTCCAGCAAGATCAGG NM_001081709
TLR2-1 F: ACATGTGTGAATGGCCTGAA
R: TTGAGAAATGGCAGTTGCAG NM_204278
TLR2-2 F: AGGCACTTGAGATGGAGCAC
R: CCTGTTATGGGCCAGGTTTA AB046533
TLR3 F: TCAGTACATTTGTAACACCCCGCC
R: GGCGTCATAATCAAACACTCC NM001011691
TLR4 F: AGTCTGAAATTGCTGAGCTCAAAT
R: GCGACGTTAAGCCATGGAAG NM_001020693
TLR5 F: CCACATCTGACTTCTGCCTTT
R: TGCACATGTTTTCTCCTAGGT NM_001024586
TLR7 F: CCTGACCCTGACTATTAACCATT
R: CGTAAAGTAGCAGGAAGACCC NM_001011688
TLR15 F: GTTCTCTCTCCCAGTGGGTGAAATAGC
R: GTGGTTCATTGGTTGTTTTTAGGAC NM_001037835
TLR21 F: TGCCCCTCCCACTGCTGTCCACT
R: AAAGGTGCCTTGACATCCT NM_001030558
IL-1β F: GTGAGGCTCAACATTGCGCTGTA
R: TGTCCAGGCGGTAGAAGATGAAG NM_204524
IL-6 F: AGAAATCCCTCCTCGCCAAT
R: AAATAGCGAACGGCCCTCA NM_204628.1
TNFSF15 F: CCTGAGTTATTCCAGCAACGCA
R: ATCCACGAGCTTGATGTCACTAAC NM_001024578
CXCLi2 F: GGCTTGCTAGGGGAAATGA
R: AGCTGACTCTGACTAGGAAACTGT AJ009800
IFN-α F: ATCCTGCTGCTCACGCTCCTTCT
R: GGTGTTGTGGTGTCCAGGATG XM_004937096
IFN-β F: GCTCTCACCACCACCTTCTC
R: GCTTGCTTCTTGTCCTTGCT NM_001024836
RPS17 F: AAGCTGCAGGAGGAGGAGAGG
R: GGTTGGACAGGCTGCCGAAGT NM_204217

F, forward; R, reverse.