Table 1. Primer sequences of TLRs, cytokines, and RPS17 for PCR.
Target genes | Sequences 5′-3′ | Accession No. |
---|---|---|
TLR1-1 | F: AGGTTGGACTTCTTATTGAGGCATAC | |
R: AGATGAATCCCAAACTAGCAGAAAAA | NM_001007488 | |
TLR1-2 | F: AGTCCATCTTTGTGTTGTCGCC | |
R: ATTGGCTCCAGCAAGATCAGG | NM_001081709 | |
TLR2-1 | F: ACATGTGTGAATGGCCTGAA | |
R: TTGAGAAATGGCAGTTGCAG | NM_204278 | |
TLR2-2 | F: AGGCACTTGAGATGGAGCAC | |
R: CCTGTTATGGGCCAGGTTTA | AB046533 | |
TLR3 | F: TCAGTACATTTGTAACACCCCGCC | |
R: GGCGTCATAATCAAACACTCC | NM001011691 | |
TLR4 | F: AGTCTGAAATTGCTGAGCTCAAAT | |
R: GCGACGTTAAGCCATGGAAG | NM_001020693 | |
TLR5 | F: CCACATCTGACTTCTGCCTTT | |
R: TGCACATGTTTTCTCCTAGGT | NM_001024586 | |
TLR7 | F: CCTGACCCTGACTATTAACCATT | |
R: CGTAAAGTAGCAGGAAGACCC | NM_001011688 | |
TLR15 | F: GTTCTCTCTCCCAGTGGGTGAAATAGC | |
R: GTGGTTCATTGGTTGTTTTTAGGAC | NM_001037835 | |
TLR21 | F: TGCCCCTCCCACTGCTGTCCACT | |
R: AAAGGTGCCTTGACATCCT | NM_001030558 | |
IL-1β | F: GTGAGGCTCAACATTGCGCTGTA | |
R: TGTCCAGGCGGTAGAAGATGAAG | NM_204524 | |
IL-6 | F: AGAAATCCCTCCTCGCCAAT | |
R: AAATAGCGAACGGCCCTCA | NM_204628.1 | |
TNFSF15 | F: CCTGAGTTATTCCAGCAACGCA | |
R: ATCCACGAGCTTGATGTCACTAAC | NM_001024578 | |
CXCLi2 | F: GGCTTGCTAGGGGAAATGA | |
R: AGCTGACTCTGACTAGGAAACTGT | AJ009800 | |
IFN-α | F: ATCCTGCTGCTCACGCTCCTTCT | |
R: GGTGTTGTGGTGTCCAGGATG | XM_004937096 | |
IFN-β | F: GCTCTCACCACCACCTTCTC | |
R: GCTTGCTTCTTGTCCTTGCT | NM_001024836 | |
RPS17 | F: AAGCTGCAGGAGGAGGAGAGG | |
R: GGTTGGACAGGCTGCCGAAGT | NM_204217 |
F, forward; R, reverse.