Table 1. Composition of feeding mixtures for broilers.
| Ingredient (%) | Mixtures 22nd–42nd day | |||
|---|---|---|---|---|
| P1 | P2 | P3 | P4 | |
| Corn | 60.78 | 60.04 | 60.29 | 60.24 |
| Animal wheat flour | 5.00 | 5.00 | 5.00 | 5.00 |
| Soybean cake | 23.85 | 23.85 | 23.85 | 23.85 |
| Animal yeast | 2.50 | 2.50 | 2.50 | 2.50 |
| Methionine DL | 0.12 | 0.12 | 0.12 | 0.12 |
| Lysine 99 % | 0.12 | 0.12 | 0.12 | 0.12 |
| Sunflower oil | 3.70 | 3.70 | 3.70 | 3.70 |
| Salt | 0.25 | 0.25 | 0.25 | 0.25 |
| Na-bicarbonate | 0.20 | 0.20 | 0.20 | 0.20 |
| Limestone | 1.22 | 1.22 | 1.22 | 1.22 |
| Monocalcium phosphate | 1.61 | 1.61 | 1.61 | 1.61 |
| Organic acids | 0.40 | 0.40 | 0.40 | 0.40 |
| Premix with phytase without coccidiostats | 0.25 | 0.25 | 0.25 | 0.25 |
| β-alanine | – | 0.50 | – | 0.20 |
| L-histidine | – | – | 0.25 | 0.10 |
| MgO | – | 0.24 | 0.24 | 0.24 |
| Total | 100.00 | 100.00 | 100.00 | 100.00 |
| *Chemical analysis of mixtures | ||||
| Water (g/kg) | 109 | 110 | 112 | 114 |
| Ash (g/kg) | 57 | 53 | 53 | 38 |
| Crude protein (g/kg) | 191.7 | 190.6 | 182.0 | 171.1 |
| Fat (g/kg) | 47 | 52 | 28 | 28 |
| Crude fiber (g/kg) | 38 | 39 | 40 | 32 |
| Calcium (g/kg) | 9.7 | 9.1 | 9.6 | 5.1 |
| Phosphorus (g/kg) | 6.9 | 5.8 | 5.9 | 4.3 |
| Sodium (g/kg) | 2.1 | 2.0 | 1.9 | 1.0 |
| Sugar | 46.6 | 47.7 | 39.0 | 39.6 |
| Starch | 371.6 | 367.9 | 401.8 | 442.1 |
| ME (MJ/kg) | 12.98 | 12.98 | 12.98 | 12.98 |
Treatments: P1=control; P2=0.5% β-alanine+0.24% MgO; P3=0.25% L-histidine+0.24% MgO; P4=0.20% β-alanine+0.10% L-histidine+0.24% MgO; a, b, P<0.05; SEM=standard error of the treatment.
Composition of premix in 1 kg of mixture: vit. A (E672) 13,000 IE; vit D3 (E671) 3,000 IE; vit E (DL-α tocopherol) 35 mg; vit K3 2.5 mg; vit B1 3 mg; vit B2 6 mg; nicotinamide 40 mg; calcium pantothenate 12 mg; vit. B6 5 mg; vit. B12 0.02 mg; biotin 0.18 mg; choline chloride 500 mg; folic acid 1 mg; iodine (E2 potassium iodide) 1 mg; iron (E1) 50 mg; copper (E4 copper sulfate-pentahydrate) 15 mg; manganese (E5, manganese sulfate, hid.) 80 mg; zinc (E6 zinc sulfate, hid.) 60 mg; cobalt 0.1 mg; selenium (E8 sodium selenite) 0.2 mg; antioxidant BHT (E321) 100 mg; phytase Ronozyme P (E1614 I) 500 FYT; organic acids 3,000 mg.
Chemical analysis of mixtures was performed by following referential methods: M-2 (HRN ISO 6496:2001), M-3 (HRN ISO 5984:2004), M-4 (HRN EN ISO 5983-2:2010), M-5 (HRN ISO 6492:2001), M-6 (HRN EN ISO 6865:2001), M11, M12 (HRN ISO 6491:2001), and M-13 (HRN ISO 7485:2001).