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Effects of IGF-I on the LC3-II content (A) and mRNA expression of atrogin-1/MAFbx and autophagy-related genes (LC3B, GABARAPL1, ATG12, and cathepsin B) (B) in chick myotube cultures. Cells were incubated for 3 h in serum-free M-199 medium with IGF-I (100 ng/ml). Data are expressed as the mean±SD (n=6). Values are significantly different at ** P<0.01.
