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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Pharmacol Biochem Behav. 2018 Sep 18;175:89–100. doi: 10.1016/j.pbb.2018.09.007

Fig. 3.

Fig. 3.

Co-immunoprecipitation of mGlu2 and 5-HT2A in the rat brain. (A) Validation of the mGlu2 (07-261) antibody. Under the low reducing conditions this antibody primarily detected mGlu2 receptor dimer (◁; ~220 kDa) in cortical lysates from rat (R) and wild-type mice (WT) that was absent in mGlu2 knock-out (ko) mice. (B) A band corresponding to mature rat 5-HT2A receptors (◀; ~75 kDa) as detected in the PRh lysates immunoprecipitated with mGlu2 (07-261) antibody. This band was not detected in the mPFC and dHPC (not shown) tissue lysates. mGlu2xx – cross-linked mGlu2 receptors. IgG HC – heavy chain of the denatured mGlu2 antibody used for immunoprecipitation.