Table 1. Primers used for PCR analysis of the expression of cytokine receptors and eggshell formation-related genes.
Target genes | Primer sequence (5′-3′) | Genbank accession no. |
---|---|---|
CaBP-D28K | F-ATGGATGGGAAGGAGCTACAA | M14230 |
R-TGGCACCTAAAGAACAACAGGAAAT | ||
PMCA1 | F-CTGCACTGAAGAAAGCAGATGTTG | XM_416133 |
R-GCTGTCATATACGTTTCGTCCCC | ||
PMCA2 | F-TTACTGTACTTGTGGTTGCTGTCCC | XM_001231767 |
R-GGTTGTTAGCGTCCCTGTTTTG | ||
CA2 | F-ATCGTCAACAACGGGCACTCCTTC | NM_205317 |
R-TGCACCAACCTGTAGACTCCATCC | ||
SLC26A9 | F-GCCTCTTCGATGAGGAGTTTGAG | XM_425821 |
R-CTGACCCCACCAAGAACATCAG | ||
IL-6 | F-AGAAATCCCTCCTCGCCAAT | NM_204628.1 |
R-AAATAGCGAACGGCCCTCA | ||
RPS17 | F-AAGCTGCAGGAGGAGGAGAGG | NM_204217 |
R-GGTTGGACAGGCTGCCGAAGT |
F, forward; R, reverse