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| Criteria | Threshold |
|---|---|
| ReadRankPosSum (RRPS) | RRPS < -8 |
| Quality by depth (QD) | QD < 5 |
| Read depth (DP) | DP < 10 |
| Fisher’s exact test p-value (FS) | FS > 60 |
| Mapping Quality (MQ) | MQ < 40 |
| SnpCluster | 3 SNPs in 35bp |
| Mann-Whitney Rank-Sum (MQRankSum) | MQRankSum < -12.5 |
| Alternative allele supporting read depth | ALTreads < 5 |
| Alternative allele ratio (Het) | aa / t ≤ 0.10 |
