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. 2019 Sep 23;14(9):e0218583. doi: 10.1371/journal.pone.0218583

Fig 2. Early bolting phenotypes observed in the T1 and T2 transgenic plants.

Fig 2

(A) Early bolting phenotype in some T1 transgenic plants. Transgenic plants were selected on antibiotic-containing 1/2 MS plates, and ~5-day-old transgenic seedlings were transferred into soil pots and grown in a growth room. As a control, seeds of Col wild type were germinated on 1/2 MS plates, and seedlings ~5-day-old were transferred into soil pots. Pictures were taken 10 days after the transfer. (B) Bolting time of the T1 transgenic plants. The date of bolting for the plants was recorded, and days after the transfer were calculated. For Col wild type plants, n = 11. For transgenic plants, n = 53. (C) Bolting time of the T2 transgenic plants from 2 independent T1 lines. T2 seeds were sown directly into soil pots and growth room. The date of bolting for the plants was recorded, and bolting time was calculated. Data represent mean ± SD of 9–40 plants.