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. 2019 Sep 23;14(9):e0218583. doi: 10.1371/journal.pone.0218583

Fig 3. AITR1 editing status in T1 transgenic plants.

Fig 3

(A) PCR amplification of AITR1 coding sequence in the T1 transgenic plants. DNA was isolated from leaves collected from Col wild type and individual T1 transgenic plants, and PCR was used to amplify coding sequence of AITR1. Amplification of ACT2 was used as a control. Picture is image of PCR results for Col wild type and T1 transgenic plants lines 1 to 15, showing the 2 PCR product bands obtained in line 14, and 1 band for Col and other lines. M, 250 bp DNA maker. (B) AITR1 editing status in sequenced individual T1 transgenic plants. PCR products were recovered from gel and sequenced. Sequencing results were examined and aligned with coding sequence of AITR1 to check the editing status in the T1 transgenic plants. Early bolting lines were numbered sequentially, whereas lines normal bolting plants were numbered according to their initial location in the tray with a letter and a number. wt, not edited, -/+, edited but heterozygous, ins, homozygous or biallelic editing with nucleotide insertions as indicated, N/A, not sequenced.