Fig 5. T2 plants with normal bolting time are transgene-free plants.

DNA was isolated from leaves collected from 4–6 individual normal bolting T2 plants for each of the 4 lines, and PCR was used to amplify Cas9 fragment. For each line, DNA was also isolated from two early bolting plants, and used a positive control for Cas9 amplification. PCR amplification of ACT2 was used as a control. M, 250 bp DNA maker.