Table 2.
Gene name | Primers (5ʹ—3ʹ) |
---|---|
SLIT2 | FORWORD: AGCCTTGTCACACTTAGCGATTGG REVERSE: ACAGCGTGCAATTCCAGGTTCC |
ROBO2 | FORWORD: TTGGAGCAAGTTCACGGGAG REVERSE: TAAGCCGCTCTGTTAGTCGG |
ROBO1 | FORWARD: CCTTTTTCACTGACGCTGATTT REVERSE: GATCACAACAGCACTCATGTTG |
CDC42 | FORWARD: CAGACTACGACCGCTAAGTTAT REVERSE: CAGCAGTCTCTGGAGTAATAGG |
RAC2 | FORWARD: CGAGAAGCTGAAGGAGAAGAAG REVERSE: GAGTGCAGAACATTCCAAGTAC |
B-ACTIN | FORWARD: GTGCTATGTTGCTCTAGACTTCG REVERSE: ATGCCACAGGATTCCATACC |
Abbreviations: SLIT2, slit guidance ligand 2; ROBO, roundabout guidance receptor; RAC2, Rac family small GTPase 2; CDC42, cell division cycle 42; B-ACTIN, actin beta.