Table 2.

Biochemical Inclusion Criteria for the Endocrine and Metabolic Responses on the Overtraining (EROS) Study

Measure	Range Required for Inclusion	Diseases Excluded by the Criteria	Assay Method
Total testosterone	>200 ng/dL	Hypogonadism	Chemiluminescence assay
Thyroid-stimulating hormone	<5 μIU/mL	Primary hypothyroidism	Chemiluminescence assay
Creatinine (and calculated estimated glomerular filtration rate)	<1.5 mg/dL (>60 mL/min)	Renal impairment	Jaffe enzymatic assay
Creatine kinase	<5000 U/L	Rhabdomyolysis, other myositis	Calorimetric activity assay; International Federation of Clinical Chemistry
Erythrocyte sedimentation rate	<25 mm/h	Inflammation and other disorders (high negative predictive value)	Automated spontaneous sedimentation method
Ultrasensitive C-reactive protein	<3 mg/dL	Infections, inflammation, cardiovascular risk	Latex-intensified immunoturbidimetry
Hematocrit	36%–54%	Anemia from several causes, polycythemia	Automated assay
Neutrophils	1000–9000/mm3	Infections, aplasia, neutropenic disorders	Automated assay
Alanine aminotransferase	<50 U/L	Liver dysfunctions	Calorimetric activity assays
Aspartate aminotransferase	<50 U/L	Liver dysfunctions, myolysis	Calorimetric activity assays
Vitamin B12	>180 pg/mL	Neuropsychiatric symptoms from vitamin B12 deficiency	Chemiluminescence assay
Fasting glucose	<100 mg/dL	Prediabetes and diabetes	Enzymatic assay of hexokinase