Fig 1. Schema of the neuropeptide identification strategy using a combination of peptide-spectrum matching against a dataset of in silico cleaved neuropeptide sequences extracted from putative neuropeptide precursors from the Nematostella protein database.

Amino- and carboxyterminal motifs were used to scan the Nematostella protein database for neuropeptide precursor candidates, from which the peptides sequences were cleaved in silico. Peptide sequences were then exported into a target database for MS/MS spectral searching.