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. Author manuscript; available in PMC: 2019 Sep 23.
Published in final edited form as: Nat Struct Mol Biol. 2014 Jan;21(1):2–3. doi: 10.1038/nsmb.2742

Figure 1.

Figure 1

AFM analysis of recombinant CENP-A and H3 octameric nucleosomes shows no appreciable difference in size. (a) Box plot representing AFM height measurements of reconstituted recombinant CENP-A (green) and H3 (red) nucleosomes and extracted HeLa nucleosomes (gray). Auto, automated analysis; X-link, samples cross-linked with 0.01% glutaraldehyde; single apostrophe, histones from J. Ottesen’s laboratory; double apostrophe, histones from A. Straight’s laboratory. P values from Kolmogorov–Smirnov test for each pair are indicated. The bottom of the box indicates the 25th percentile, the top of the box indicates the 75th percentile, and the horizontal line inside the box indicates the median. The bottom and top whiskers indicate 10th and 90th percentile, respectively, and the black dots indicate outlying values. (b) Box plot of AFM diameter measurements of nucleosomes represented in a. (c) AFM height measurements of the same sample over time. Results from Cse4 octamers cross-linked with 0.01% glutaraldehyde, deposited on APS surface and scanned over the course of 10 d are shown. Error bars, s.d. Raw data are summarized in Supplementary Data Set 1.