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. 2019 Sep 3;11(17):6983–6998. doi: 10.18632/aging.102232

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Copyright © 2019 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Osteogenesis is suppressed upon upregulation of miR-126a-3p in hADSCs. (A) The expression level of miR-126a-3p was detected using qRT-PCR in transduced hADSCs with lentivirus overexpressing miR-126a-3p (LV-126a) or the negative control (LV-NC). (B) The mRNA levels of osteogenic-related genes were detected by qRT-PCR assay in osteogenic-induced cells on day 6. (C) Western blot assays analyzed the protein levels osteogenic-related genes in osteogenic-induced cells. (D, E) ALP staining and ALP activity analyses indicated early differentiation on day 6 of osteogenic differentiation. (F) Alizarin red staining was performed to detect calcium salt deposits on day 12. Scale bars: 200 μm. Quantitative data are presented as the mean ± S.D. (n =3). *P<0.05; **P<0.01; ***P<0.001.