Skip to main content
. 2019 Sep 10;11(17):7242–7256. doi: 10.18632/aging.102259

Figure 6.

Figure 6

Downregulation of Idh2 promotes senescence in mouse embryonic fibroblasts (MEFs) through suppressing Cdk2. (A) Wild type and Idh2 knockout MEFs were incubated with propidium iodide for 15 min at 37°C. Cell cycle was detected with flow cytometry. (B) Cyclin-dependent kinase protein levels were detected with western blot analysis in wild type and Idh2 knockout MEFs. The following antibodies were used for detection: anti-p21, anti-Cdk1, anti-Cdk2, anti-Cdk4, anti-Cdk6, and anti-β-actin. Data are expressed as means ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.