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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Kidney Int. 2019 Jul 10;96(4):883–889. doi: 10.1016/j.kint.2019.06.016

Figure 2. KIRREL1 mutants mislocalize in human podocytes.

Figure 2.

(A) Schematic domain distribution, position of Flag tag and mutations in the KIRREL1 protein. (B) Western blot analysis showing the expression of the wild type control and KIRREL1 mutants plasmid constructs as indicated. (C) Unpermeabilized wild type human podocytes, human podocytes stably expressing flag tagged wild type KIRREL1 and flag tagged KIRREL1 mutants p.Ser573Leu and p.Arg440Cys were stained with Flag antibody, that labeled Flag-KIRREL1 extracellularly. Their membrane localization was evaluated by immunofluorescence microscopy using a confocal microscope. KIRREL1 mutants p.Ser573Leu and p.Arg440Cys show mislocalization compared to wild type control in cultured human podocytes cell lines. (Scale bars: 25 μm).