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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Stroke. 2019 Aug 15;50(10):2902–2911. doi: 10.1161/STROKEAHA.119.025940

Figure 1.

Figure 1.

β-estradiol attenuates acidosis-induced cytotoxicity. A and B, Viability and cytotoxicity in NS20Y cells and mouse cortical neurons were measured at 24 h after acid treatment by MTT and LDH assays. C, Representative phase-contrast images showing NS20Y cells taken after treatment with the indicated solutions (×200 magnification). D, Analysis of acid-induced injury with fluorescein diacetate (FDA, alive) and propidium iodide (PI, dead) (×200 magnification). ***P<0.001 versus control (pH 7.4). #P<0.05, ##P<0.01, ###P<0.001 versus pH 6.0.