β-estradiol down-regulates ASIC1a protein expression. A, ASIC1a protein expression in NS20Y cells were measured after treatment with β-estradiol (1 μmol/L) for 48 h. B, ASIC1a protein expression in NS20Y cells were measured after 10 nmol/L–1 μmol/L β-estradiol treatment for (24 – 72 h). C, ASIC1a protein expression in cultured mouse cortical neurons were measured after 1 μmol/L β-estradiol treatment for 48 h. D, ASIC1a protein expression in NS20Y cells were measured after 1 μmol/L α-estradiol treatment for 48 h. *P<0.05, **P<0.01, ***P<0.001 versus control.