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ER antagonist ICI 182 780 suppresses β-estradiol-induced decrease of ASIC1a protein expression in NS20Y cells. A, Expression of ERα and ERβ protein in NS20Y cells. MCF-7, a breast cancer cell line, was used as a positive control. B, ASIC1a protein expression was analyzed by Western blot after treatment with ICI 182 780 with or without β-estradiol. ***P<0.001 versus control. ###P<0.001 versus β-estradiol.
