A-B. Mutations are presented in tables with each row representing a mutation and each column representing a sample. Blue color indicates a mutation call, with variant allele frequencies in each cell of the table. Both depicted IPMNs have multiple mutations called in a later-driver gene. A. IPC16, a high-grade IPMN, has four distinct RNF43 mutations. B. IPC20, a high-grade IPMN, has two different RNF43 mutations and four distinct TP53 mutations. C-D. The 20 IPMNs are represented around the perimeter of the circle in clockwise numerical order – grade of dysplasia/analysis-type for each IPMN is indicated by the colored key. Only heterogeneous mutations are displayed (not all mutations). Each colored rectangle represents a heterogeneous mutation in a given gene, which is indicated by colored key. The heterogeneous mutations in each IPMN are presented by gene in low-grade and whole lesion analysis of high-grade IPMNs (C) and low-grade and high-grade only analysis of high-grade IPMNs (D). Note: Only genes with mutations in at least two IPMNs were included in C-D.