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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Methods. 2019 May 9;167:117–123. doi: 10.1016/j.ymeth.2019.05.004

Fig. 2.

Fig. 2.

An illustration of the FP-based screening and binding assay. Upon excitation by polarized light, (a) a free fluorescent pre-miRNA binder emits depolarized light; (b) when complexed to pre-miRNA, it gives polarized light due to reduced tumbling; (c) a strong pre-miRNA binder replaces the fluorescent binder from the complex, resulting in decreased polarity of the light emitted.