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. Author manuscript; available in PMC: 2020 Sep 24.
Published in final edited form as: Circulation. 2019 Aug 12;140(13):1100–1114. doi: 10.1161/CIRCULATIONAHA.119.040232

Figure 5. Local sympathetic nerves drive rhythmic inflammatory responses.

Figure 5

(A) In vivo quantification of adherent leukocytes after TNF-α stimulation in carotid artery and jugular vein after 6-OHDA treatment. Data are normalized to ZT1 control levels; n = 5-8 mice, Student’s t-test. (B) In vivo quantification of adherent leukocytes after TNF-α stimulation in carotid artery and jugular vein after unilateral surgical denervation of the superior cervical ganglion (SCGx). Data are normalized to ZT1 control levels; n = 5-12 mice, Student’s t-test. (C-D) Q-PCR analyses of cell adhesion molecules after TNF-α stimulation in carotid artery (C) and jugular vein (D) of SCGx mice. Data are normalized to ZT1 control levels; n = 4-7 mice, Student’s t-test with Welch’s correction. (E) In vivo quantification of adherent leukocytes after TNF-α stimulation in carotid artery and jugular vein of Adrb2−/− mice. Data are normalized to ZT1 control levels; n = 6-9 mice, Student’s t-test. (F) In vivo quantification of adherent leukocytes after TNF-α stimulation in carotid artery and jugular vein after 5 d (chronic) treatment with a β2 adrenergic receptor antagonist. Data are normalized to ZT1 control levels; n = 4-7 mice, Student’s t-test with Welch’s correction. (G) Q-PCR analyses of cell adhesion molecules after TNF-α stimulation in carotid artery and jugular vein after chronic (5 d) or acute (2 h) treatment with a β2-adrenergic receptor antagonist. Data are normalized to ZT1 control levels; n = 5-6 mice, Student’s t-test with Welch’s correction. (H) In vivo quantification of adherent leukocytes after TNF-α stimulation in carotid artery and jugular vein after 2 h (acute) treatment with a β2-adrenergic receptor antagonist. Data are normalized to ZT1 control levels; n = 6-11 mice, Student’s t-test. (I) Q-PCR analyses of cell adhesion molecules after TNF-α stimulation in carotid artery and jugular vein after acute (2 h) treatment with a β2-adrenergic receptor agonist; n = 5-6 mice, Student’s t-test with Welch’s correction. *p < 0.05, **p < 0.01.