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. 2019 Sep 23;9:13713. doi: 10.1038/s41598-019-50261-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hydrocarbon production in cultures of E. coli expressing FAP or FAP and thioesterase. Time course of hydrocarbon production. Cells were grown in batch culture under constant light illumination. Chlorella variabilis FAP (FAP) and Umbellularia californica thioesterase (Tes) were expressed in E. coli. Hydrocarbons were quantified in the harvested cells and in the gas phase of the culture using the hydrocarbon trap (Fig. 2). Total refers to cells plus gas phase. Data represent the mean ± SD of three independent experiments using three different bacterial cultures.