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. 2019 Sep 23;9:13677. doi: 10.1038/s41598-019-50216-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ITCs induce the stringent alarmone synthesis in some but not all tested Gram(−) (A) and Gram(+) (B) bacteria. The synthesis of ppGpp and pppGpp, and level of GTP were assessed by culturing bacteria in the presence of [³²P] orthophosphoric acid followed by cell lysis and nucleotide separation by thin-layer chromatography. SHX served as positive control.