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. 2019 Sep 23;9:13700. doi: 10.1038/s41598-019-50105-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Coordination of T1Cu: (a) in the structure of WT:FECN (PDB ID 6I3J, carbon green), (b) in W396A:FECN (PDB ID 6I3K, carbon light blue), and (c) in W396F (PDB ID 6I3L, carbon pale orange). Distances are given in Ångströms with 0.01 Å precision to support the discussion of the T1Cu environment changes. Values in parentheses are for chain B of the corresponding structure. T1 copper is shown as orange sphere. The Phe396 side chain in the structure W396F adopts two conformations marked conf. A and conf. B. Molecular graphics were created using PyMOL (Schrödinger, LLC).