Table 1.
Validation results for measuring zonisamide concentrations in human serum by the HPLC method
| Zonisamide concentrations measured by HPLC (µg/mL) | ||||
|---|---|---|---|---|
| LLOQ | QC A | QC B | QC C | |
| 1 | 3 | 30 | 75 | |
| Intra‐day | ||||
| Mean (µg/mL) | 1.04 | 3.02 | 30.18 | 73.17 |
| Accuracy (%) | 3.73 | 0.59 | 0.59 | −2.44 |
| Precision (%CV) | 1.17 | 1.33 | 1.86 | 1.57 |
| Inter‐day | ||||
| Mean (µg/mL) | 0.94 | 2.93 | 28.81 | 70.25 |
| Accuracy (%) | −5.78 | −2.45 | −3.98 | −6.33 |
| Precision (%CV) | 10.27 | 4.95 | 6.39 | 9.00 |
Abbreviations: CV, coefficient of variation; HPLC, high‐performance liquid chromatography; LLOQ, lower limit of quantitation; QC, quality control.