Fig. 1.

Comparison of separate lipid species from SchuS4 for anti-inflammatory activity. a Separation of crude SchuS4 lipids by TLC. Increasing concentrations of SchuS4 lipids were spotted onto silica plates and separated using ethyl acetate: MeOH (3: 2) and Chl: MeOH:H₂O (7: 3: 0.25) into 4 distinct bands. b Each band (1–4) was scraped from the silica plate, processed to remove contaminating silica, and added to human dendritic cells (hDC) at 2 µg/mL (3–4 wells/group), followed by stimulation with E. coli LPS (5 ng/mL). Control samples for bands consisted of scrapings from the silica plate adjacent to each sample band. hDC were also treated with 10 µg/mL of crude lipid (CL). Control for crude lipid consisted of EtOH diluted similarly to the lipid. After 24 h, supernatants were assessed for IL-12p40 and TNF-α. Values from the control sample and corresponding band were analyzed using an unpaired, two-tailed, Student's t test. A–C indicate individual donors. Error bars represent standard deviation (SD).