Fig. 6.

PE chain length of 24: 0/–10: 0 is required for inhibition of inflammatory responses in host cells. Individual PE consisting of either 24: 0/–10: 0 (PE2410) or 18: 1/–16: 0 (PE1816) or PE liposomes comprising PE2410 and PC 24: 0/–24: 0 (PEPC2410) were added to mouse bone marrow-derived macrophages (BMDM) (a) or human dendritic cells (hDC) (b) (3–4 wells/group) at the indicated concentrations and allowed to incubate overnight. hDC or BMDM were then stimulated with E. coli LPS (5 ng/mL) or R848 (5 ng/mL), and supernatants were collected the next day to assess IL-12p40. Mock-treated controls (–) were treated with 5% dextrose in water diluted similarly to wells containing 10 µg/mL lipid. BMDM data represent 3 experiments of similar design. A–C indicate individual donors. Data were analyzed using one-way-ANOVA followed by Tukey's multiple comparison analysis. * p < 0.05 compared to mock-treated controls and cells treated with 0.1 µg/mL lipid; ** significant compared to mock treated controls; ns, not significant. Error bars represent SD.