Fig. 3.

A. fumigatus is more efficiently recognized and phagocytosed than A. nidulans. a Two representative frames from live cell-imaging videos illustrating the measurement of migration distance (left panel) and definition of contact between an A. fumigatus conidium and a J774A.1 cell (right panel). b Percentage of J774A.1 cells (left panel) and BMDMs (right panel) that show active migration to A. fumigatus or A. nidulans resting conidia or swollen conidia. c Mean distance (μm) measured using Volocity software from the moment of initiation of migration by J774A.1 cells (left panel) and BMDMs (right panel). d Migration speed (μm/min) measured by Volocity software from the moment of movement initiation to the moment of contact between J774A.1 cells (left panel) and BMDMs (right panel) and conidia. All bars and dot plots represent the mean ± SEM, derived from 2 independent experiments with 4 videos per experiment for J774A.1 cells and 2 independent experiments with 2 and 4 videos for BMDMs. Means were compared for significance using the Mann-Whitney U test; * p < 0.05, ****p < 0.0001