Fig. 3.
SeNOS expression profile. a Variation of SeNOS expression in all developmental stages: egg, larval instars (L1-L5), pupa (P), and adult (A). b Variation of SeNOS expression in 4 larval tissues, hemocyte (HC), fat body (FB), midgut (GUT), and epidermis (EPD). c Inducible expression of SeNOS following bacterial injection. Heat-killed X. hominickii (HK-Xh; 1 × 105 bacterial cells per larva) were injected into L5 larva. After the indicated time intervals, FB was collected and processed for measuring mRNA levels. Each treatment had 3 biologically independent replicates. Different letters above the SD bars indicate significant difference among means at type I error = 0.05 (LSD test). A ribosomal gene, RL32, was used as the stably expressed reference gene for RT-PCR (upper panel) and qPCR (lower panel).