Fig. 2.

p33 impairs DAMP-mediated NET release. a Representative immuno-fluorescent micrographs display the results shown in b and c. NET structures were visualised with MPO (green) and DAPI (blue). Scale bar, 50 µm. b, c Human neutrophils were treated for 3 h with 5 µm LL-37 and 50 µg/ml CTHs, respectively. Recombinant p33 was added simultaneously in a 2:1 ratio. 3 independent experiments +/- SEM. Ctr, control. ** p ≤ 0.01, **** p ≤ 0.0001.