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. 2017 Oct 17;10(1):30–43. doi: 10.1159/000480386

Fig. 3.

Fig. 3

a Representative fluorescence microscopy images of the PMA-triggered NET release. Histone H1, green; nuclei, blue. Scale bar, 50 µm. b Inhibitory effect of 100 µg/mL p33 on human PMNs incubated with 25 nM PMA for 3 h. c, d Scanning electron micrographs of 25 nM PMA-induced NETs in the presence or absence of 100 µg/mL p33. ×1,850. Scale bar, 30 µm. e, f Representative fluorescence images of NET formation induced by S. pyogenes AP1. Histone H1, green; nuclei, blue. Scale bar, 30 µm. g The addition of 100 µg/mL p33 shows significant inhibition of S. pyogenes-induced NET release. Data is for 3 independent experiments; unpaired one-tailed Student t test +/- SEM. *** p < 0.001, **** p ≤ 0.0001.