Fig. 1.

STING is important for dendritic cell activation. Bone marrow-derived DCs from STING^–/–, cGAS^–/–, or wild-type (WT) mice were infected with M. tuberculosis (Mtb) H37Rv (MOI 3: 1) or stimulated with Mtb DNA (1 µg/mL) during 24 h. a The expression of Ifn-β was analyzed by real-time qPCR using IFN-β and β-actin primers. All data were normalized to β-actin gene expression. b, c Levels of CXCL10 and IL-12p40 were measured in the cell supernatants by ELISA. d, e Mean fluorescence intensity (MFI) of CD86 and CD40 expression was analyzed by flow cytometry. Data are shown as mean values ± SD and are from 1 experiment representative of 3 independent experiments. Statistically significant difference in relation to WT: * p < 0.05; ** p < 0.01; *** p < 0.001 (two-way ANOVA followed by the Bonferroni post hoc test).