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. 2019 Sep 24;39(9):BSR20192196. doi: 10.1042/BSR20192196

Figure 5. Anti-HIV-1 activity of C34 and N36 peptides and the trimer C34 and trimer N36 in a single infectivity assay.

Figure 5

HeLa CD4-CCR5/CXCR4-LTR/β-gal cells were infected for one viral cycle (20 h) with 0.1 ng of HIV-1 BaL (A,C) or VN44 (B,D). Viral replication was correlated directly with the transactivation of the Lac-Z gene by the early translated HIV-1 Tat gene. β-galactosidase activity was visualized by incubating cells in the presence of the substrate X-Gal which stained the cells blue after X-Gal degradation by the action of the LTR-driven expression of β-galactosidase. Experiments were performed in triplicate and repeated three times. A representative experiment is shown as mean ± standard deviation. *, P<0.05; **, P<0.001; ***, P<0.0001.