Figure 1.

Effects of social isolation in adolescence on cortico-striatal CNPase during versus after isolation. Experimental timelines are inset, and shading highlights cortical regions. A, Mice were socially housed or isolated starting at P31. CNPase was measured, given existing evidence that social isolation impoverishes oligodendrocyte morphology, and effects are persistent, despite social reintegration (Makinodan et al., 2012). At P39, CNPase did not differ between groups (n = 8–9/group). B, Isolated mice were reintegrated into social groups at P60. At P82, previously isolated mice had decreased CNPase in multiple cortico-striatal subregions (n = 7–12/group). Mean ± SEM, *p < 0.05 versus socially housed control, replicated in two independent cohorts. S1, somatosensory cortex; M2, secondary motor cortex; AI, agranular insula; VLO, ventrolateral OFC; MO, medial OFC; PL, prelimbic PFC; IL, infralimbic cortex; Cg1, primary cingulate cortex; Cg2, secondary cingulate cortex; DLS, dorsolateral striatum; DMS, dorsomedial striatum; Nac, nucleus accumbens; aca, anterior commissure.