Figure 2.

Cell cultures were tested to support the modified methodology. A) Growth rate of human gingival fibroblast cells (HGFCs) was identified from the 5-13th day. B) MTT formazan absorbance played a role as a function of cellular proliferation for a range of cell concentrations. C) Cell viability test on HGFC with porphyromonas gingivalis (PG-LPS) at various concentrations (0.5, 1, 5, and 100 µg/mL) after 48 hours using cell counting kit-8. Based on the one-way analysis (ANOVA) the result showed that 100 µg/mL porphyromonas gingivalis treatment were significantly different compared to control. *** and ****p<0.0001