Figure 4.

A) The released differential growth factors (PDGF-AA and TGF-β1) were quantified using Enzyme-linked Immunosorbent Assay method at various time points (1-3, 24, 48, 72 hours, and 10 days). B) Cumulative release of differential growth factors (PDGF-AA and TGF-β1) in term of concentration (pg/mL) at different time points. Based on the two-way analysis (ANOVA), **p<0.002 determines a statistically significant higher release than all the other groups, #p<0.002 determines a statistically significant lower release than all the other groups. PDGF-AA - platelet derived growth factor, TGF-b1 - transforming growth factors-beta 1, GF - growth factor