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. 2019 Aug 14;18(4):4064–4073. doi: 10.3892/ol.2019.10741

Figure 1.

Figure 1.

miR-361 is upregulated in 5-FU-resistant HCT116 and HT29 cells. (A) Parental and 5-FU-resistant HCT116 and HT29 cells were treated with 5-FU at the indicated concentrations for 48 h and subjected to a CCK-8 assay. Cell viability was presented as the relative value normalized to the 0 µg/ml group (n=6; **P<0.01, parental vs. resistant cells; ##P<0.01, the viability of resistant cells at 20 µg/ml vs. 0 µg/ml). (B) Relative differential expression of candidate miRNAs between parental and 5-FU resistant HCT116 and HT29 cells (n=3; *P<0.05, **P<0.01, vs. parental cells). (C) Overexpression of miR-361 sensitizes parental and resistant HCT116 and HT29 to 5-FU. The parental and 5-FU-resistant HCT116 and HT29 cells were transfected with an miR-361 mimic or NC, exposed to 5-FU at the indicated concentrations for 48 h and subjected to a CCK-8 assay (n=6; **P<0.01). miR/miRNA, microRNA; 5-FU, 5-fluorouracil; CCK-8, Cell Counting Kit-8; NC, negative control; Res, resistant.