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. 2019 Jul 31;18(4):3646–3654. doi: 10.3892/ol.2019.10691

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Multipotency of neurosphere cell differentiation observed under a laser confocal scanning microscope. (A-I) Cells were stained for (A-C) GFAP (red), (D-F) β-tubulin III (green) and (G-I) GALC (green) after GSC differentiation. DAPI (blue) indicates nuclei. Scale bars, 20 µm. (J-R) GSCs were stained for (J-L) GFAP (red), (M-O) β-tubulin III (green) and (P-R) GALC as control. (S) Quantification of cells positively stained by each antibody. *P<0.05. Scale bars, 50 µm. GFAP, glial fibrillary acidic protein; GALC, galactosylceramidase.