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. 2019 Sep 12;2019:8768164. doi: 10.1155/2019/8768164

Figure 3.

Figure 3

miR-154-5p directly targets Arsb. (a) A binding site for miR-154-5p in the 3′-UTR of Arsb was analyzed using miRNA target prediction databases, including miRBase, miRanda, TargetScan, and PicTar. The mutated sequence of the binging site is marked in red. (b) Luciferase activity was analyzed in HEK-293A cells 24 h after transfection with the indicated plasmids: miR-154-5p+vectors, miR-154-5p+Arsb 3′UTR, and miR-154-5p+mutated Arsb 3′UTR (n = 4). (c, d) The level of the Arsb protein was examined using Western blot analysis in the NC, miR-154-5p mimic, NCi, and miR154-5p inhibitor groups (n = 3). (e) Protein level of Arsb in sham and AngII mice explored by Western blot (n = 3). (f) Protein level of Arsb in AngII-treated primary cardiomyocytes transfected with NCi and miR-154-5p inhibitor (n = 3). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.