Table 1. Binding Data for Mtb UgpBa.
| enzyme | substrate | Kd (μM) | reference |
|---|---|---|---|
| Mtb UgpB | GPC | 3.6 ± 0.5 | this study |
| Mtb UgpB | GPS | 14.9 ± 1.6 | this study |
| Mtb UgpB | GPE | 74.7 ± 13.9 | this study |
| Mtb UgpB | GPI | 1053.2 ± 313.4 | this study |
| Mtb UgpB | GPI4P | 289.8 ± 54.1 | this study |
| Mtb UgpB | G3P | – | this study |
| Mtb UgpB | phosphocholine | – | this study |
| Mtb UgpB Y78A | GPC | – | this study |
| Mtb UgpB Y78A | GPS | – | this study |
| Mtb UgpB Y78A | GPE | – | this study |
| Mtb UgpB D102A | GPC | – | this study |
| Mtb UgpB D102A | GPS | – | this study |
| Mtb UgpB D102A | GPE | – | this study |
| Mtb UgpB Ser153Ala | GPC | 309.8 ± 56.1 | this study |
| Mtb UgpB S153A | GPS | 102.5 ± 16.4 | this study |
| Mtb UgpB S153A | GPE | – | this study |
| Mtb UgpB L205A | GPC | 161.7 ± 15.9 | this study |
| Mtb UgpB L205A | GPE | 1360 ± 210 | this study |
| Mtb UgpB W208A | GPC | – | this study |
| Mtb UgpB S272A | GPC | – | this study |
| Mtb UgpB Y345A | GPC | – | this study |
| Mtb UgpB R385A | GPC | – | this study |
| Mtb UgpB | GPC | 27.3 ± 2.0 | (9) |
| Mtb UgpB | G3P | – | (9) |
| Mtb UgpB | maltose | – | (9) |
| Mtb UgpB L205W | GPC | – | (9) |
| Mtb UgpB L205W | G3P | – | (9) |
| E. coli UgpB | GPC | 5.1 ± 0.3 | (10) |
| E. coli UgpB | G3P | 0.68 ± 0.02 | (10) |
a
(−) = no binding detected; standard deviations from at least three independent experiments. GPC: glycerophosphocholine; GPS: glycerophosphoserine; GPE: glycerophosphoethanolamine; GPI: glycerophosphoinositol; GPI4P: glycerophosphoinositol-4-phosphate.