Figure 2.

Design and cellular evaluation of second and third generation SGK PROTACs. (A) Chemical structure of compounds DAT5–12, expanding upon DAT1. (B) Chemical structure of cisSGK3-PROTAC1. (C) HEK293 cells were treated for 48 h with increasing concentrations of each PROTAC compound from 1 nM to 10 μM. Cell lysates were subjected to immunoblot analysis with the indicated antibodies, and SGK3 protein levels quantified in Image Studio Lite software. (D) HEK293 cells were treated for 48 h with increasing concentrations of each PROTAC compound from 1 nM to 10 μM. Cell lysates were subjected to immunoblot analysis with the indicated antibodies, and SGK3 and S6K1 protein levels were quantified in Image Studio Lite software.