Figure 3.

Effect of salidroside (SAL) on the mitochondrial biogenesis-associated PGC1α-TFAM signaling pathway. 2BS cells at 50PD were treated by salidroside (SAL) at various dosages or resveratrol (RES) at 10 μM for 24 h and then were harvested for western blot analysis. (a) Representative images were acquired from three different experiments. (b) Quantitative analysis of the protein levels of PGC-1α, NRF1, and TFAM. Bars represent relative protein levels counted as D₁/D₀ (the value for 50PD control was set as 1.0), where D₀ and D₁ stand for the optical density of β-actin ladder and sample ladder, respectively. The optical density for each ladder was calculated by the ImageJ software. Data were obtained from three independent experiments. ^∗P < 0.05 versus the 50PD control group.