Figure 3.

a) Effect of compound 7 on the production of HIV-1 wild-type and mutant pseudotypes as judged by supernatant p24 content. 293T cells were transfected for production of luciferase-reporter pseudotypes (as described in the Experimental Section) in the absence or in the presence of compound 7 (100 μM), and the culture supernatants containing pseudotype stocks were then analyzed by p24 ELISA. Shown are the mean values of four independent productions, with error bars depicting the standard error of the mean (SEM). The indicated p value was derived from a two-tailed paired t-test analysis. b) Refined predicted binding mode of compound 7 in the PI(4,5)P₂ binding site of HIV-1 MA. Binding site residues (L21, R22, W36, R76, T81, and K98) are represented as dark sticks, whereas compound 7 is shown in green sticks. Residues highlighted in blue are thought to be critical for compound binding. Yellow dashed lines represent hydrogen bonds.