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TUNEL assay with HRPC cells exposed to liposome-encapsulated sirolimus (LES) (identified by yellow boxes) and liposomes alone (identified by red boxes) in different doses (62.5 µg/mL, 125 µg/mL and 250 µg/mL). The images outlined with dashed white boxes show the control, the BSS/control (d1–2) and the DMSO (dimethyl sulfoxide) positive control (h1–2). Scale bars = 200 μm
