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. 2019 Sep 23;18:327. doi: 10.1186/s12936-019-2959-8

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The rMSP10 western blot. a P. falciparum Merozoite Surface Protein 10 (rMSP10) expressed in baculovirus: Lane 1: an Sf9 cell lysate from the recombinant baculovirus with the optimized pfmsp10 gene. Lane 2: The lysate of non-infected Sf9 cells. Lane 3: molecular weight protein marker (kDa). b rMSP10 purified: Eluted samples obtained from the purification process with imidazole: Lane 1: 50 mM; lane 2: 150 mM; lane 3: 200 mM; lane 4: 250 mM; lane 5: lysates from uninfected Sf9 cells; lane 6: lysates from Sf9 cells infected with the recombinant baculovirus with pfmsp10 gene; lanes 7–9: washes. Black arrows indicate rMSP10 (~ 56 kDa)