Fig. 1.

Culturing of hASCs on type 3 NFC dressing without cell adhesion coatings. SEM micrographs from a NFC dressings and b from hASCs cultured for 7 days with cell densities of 300,000 cells/cm² (300k) and 500k (n = 2). Similar morphology compared with the control cells were observed with both cell densities. Scale bars, 200 μm. Magnification, × 500. c Cell viability of hASCs with cell densities of 300k and 500k. High cell viabilities were observed especially with 300k cell density during 2-week culturing compared with the control cells cultured on plastic (all values are mean ± SEM, n = 3). d Majority of seeded cells adhered to the surface of type 3 NFC dressing within 24-h culturing. Compared with control cells, a lower number of adhered cells were observed only at 1-h time point (all values are mean ± SEM, n = 3, at 24 h time point n = 2). *p < 0.05. NFC, nanofibrillar cellulose