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. 2019 Sep 23;10:298. doi: 10.1186/s13287-019-1399-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Structure of the platelet-rich fibrin (PRF). a A cell sheet that could be fully stripped was formed after 14 days of induction with cell sheet induction medium. b Inverted microscope observation showed the intertwined arrangement and stable growth morphology of the cell sheet (× 40, bar = 200 μm). c Blood samples (10 ml) were extracted from the central auricular arteries of rabbits and immediately centrifuged at 3000 r/min for 10 min. d PRF clot between the top layer of serum containing a few cells and the bottom layer of red corpuscles. e Complete removal of the PRF clot. f Preparation of the BMSC/PRF construct. g–i Cross-sectional observation by SEM. g Horizontal view at low magnification (× 1000). h Longitudinal view at low magnification (× 2000). i Longitudinal view at high magnification (× 20,000) (red arrow: BMSCs; yellow arrow: PRF)