Fig. 2.

TUFT1 can regulate the Rab5 activation. a TUFT1 protein and mRNA expression levels were examined by western blotting and qPCR in MDA-MB-231 cells after infection with lentivirus containing TUFT1-shRNA or pLNCX2-TUFT1. b TUFT1 expression was downregulated by TUFT1-shRNA and upregulated by PLNCX2-TUFT1 in MDA-MB-231 and HCC1937 cells. Rab5-GTP expression was examined by western blotting (n = 3). c Rab5 protein and mRNA expression levels were examined by western blotting and qPCR in MDA-MB-231 cells after infection with lentivirus containing Rab5-shRNA or scr-shRNA. d Rab5-shRNA or scr-shRNA were transfected into TUFT1-overexpressing MDA-MB-231 and HCC1937 cells. Cell migration was monitored by transwell assay (n = 3). Results are presented as mean ± SD. The statistical significance was assessed by student’s t test; *p < 0.05, **p < 0.01